FIG 2.

Genome-wide CRISPR knockout and CRISPRi screens to dissect enriched genes and biological pathways in S. flexneri infection. (A) Enriched genes in the Venn diagram were filtered with a cutoff FDR of <0.25 and log₂ fold change of >1 in S. flexneri ΔvirG infection. The degree of significance of the overlap between genome-wide CRISPR knockout and CRISPRi screens is given. (B) Gene-centric visualization of the average log₂ fold change of CRISPR knockout and CRISPRi screens in S. flexneri-infected versus noninfected host cells. Selected components of TLR1/2, the pyruvate catabolism signaling pathway, and inflammasome formation are highlighted in orange, purple, and green, respectively. (C) Top enriched genes and associated biological pathways in S. flexneri ΔvirG infection. The color gradient of gene boxes represents the log₂ fold change of gene sets in genome-wide CRISPR knockout and CRISPRi screens. TCA, tricarboxylic acid.