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Single-substrate assays demonstrating the l,d-carboxypeptidase and l,d-endopeptidase activities of LdcA enzymes on stem tetrapeptides (A to L) and pentapeptides (M to X), respectively. LC-MS traces are shown for each reaction. Chemical structures of substrates (preceded by an “s”) are shown in Fig. S2. MS data are shown in Fig. S3 to S5.
