TABLE 1.
Reactions of synthetic PGNs with ApLdcA or EcLdcA, given as the percentage of product formed following treatment of different peptide (column headers) plus glycan (rows) substrates with enzyme (columns)
| Glycana | Tetrapeptide (Ala-Glu-mDap=Ala) |
Pentapeptide (Ala-Glu-mDap=Ala-Ala) |
Tetra-tripeptide (4,3) (Ala-Glu-mDap-Ala=mDap-Glu-Ala) |
|||
|---|---|---|---|---|---|---|
| ApLdcA | EcLdcA | ApLdcA | EcLdcA | ApLdcA | EcLdcA | |
| mM | 100 | 100 | 75 | 38 | 8/35 ∗4/6 |
7/22 ∗3/6 |
| aM | 100 | 100 | 84 | 41 | 18/66 | 13/29 |
| G-mM | NA | NA | 41/80 ∗0/12 |
18/56 ∗0/3 |
NA | NA |
| G-aM | 100 | 100 | 43/89 ∗0/5 |
8/25 ∗0/0 |
NA | NA |
| GMG-mM | NA | NA | 37/58 ∗0/12 |
14/51 ∗0/3 |
NA | NA |
| GMG-aM | 100 | 100 | NA | NA | NA | NA |
a
Product formation was determined after 2 h (single value) or at 2 and 24 h (values separated by “/”, respectively). Values preceded by an asterisk (∗) represent the percentage of N-acetyl muramyl l-Ala amidase product observed. For glycans, mM indicates that the MurNAc C-1 hydroxyl is replaced by β-OCH3, and aM indicates 1,6-anhydro-MurNAc. “NA” indicates where substrates were not available.