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. 2021 Dec 21;12(6):e03228-21. doi: 10.1128/mbio.03228-21

FIG 8.

FIG 8

Both Syk and EGFR are needed for phosphorylation of Tyr240 and Tyr245. (A) Syk activity is required for Y245 phosphorylation. HT1080 cells were pretreated with R406 for 1 h and treated with cGAMP for 3 h, and the cell lysates were subjected to WB with the pY245 antibody of STING. (B) Gefitinib and EGFR KO inhibited both Y245 and Y240 phosphorylations. (C) Gefitinib inhibited the phosphorylation of Y240 and Y245, as revealed by LC-MS/MS analysis. Bars show means and SD from 3 independent LC-MS/MS experiments. ***, P < 0.001 compared to the gefitinib‐pretreated group by two‐tailed Student’s t test. (D) The absence of EGFR inhibited the phosphorylation of both Y240 and Y245. STING phosphorylation was analyzed by LC‐MS/MS as described above. Bars show means and SD from 3 independent LC-MS/MS experiments. ***, P < 0.001 compared to the EGFR KD group by two‐tailed Student’s t test.