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. 2021 Dec 7;12:771499. doi: 10.3389/fphys.2021.771499

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Copyright © 2021 Kiriaev, Kueh, Morley, North, Houweling and Head.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The degree of fiber branching in extensor digitorum longus (EDL) muscles as a percentage of all fibers by categorizing them based on number of branch points. Fibers from littermate controls were omitted due to very low presence of fiber branching, particularly, less than 1%. Note this is a bar graph of all fibers counted and as such is an absolute measure, all photomicrographs in Figures 9–11 were taken from these fiber counts. A total of 11,657 fibers from 65 EDL muscles were counted: 7,119 fibers from 34 controls, and 4,538 fibers from 31 mdx muscles. For the 4-month group: n = 1,514 control n = 1,030 mdx fibers; 9-month group: n = 1,320 control n = 1,130 mdx fibers; 15-month group: n = 1,615 control n = 1,263 mdx fibers; 18-month group: n = 1,435 control n = 605 mdx fibers; and 22 month group: n = 1,235 control n = 511 mdx fibers.