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. 2021 Dec 7;12:771499. doi: 10.3389/fphys.2021.771499

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Copyright © 2021 Kiriaev, Kueh, Morley, North, Houweling and Head.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Examples of medium power light microscope images (X100) of enzymatically digested EDL muscle fibers taken from a single 22-month-old dystrophic mouse. Note the fibers have been stitched together at the same magnification from photomicrographs taken from overlapping fields of view to capture a large portion of the fiber, scale bar provided at 50 μm. Backgrounds debris from the digest process have been cleared to focus on the muscle fiber and arrows mark areas where fiber branching has occurred (To see examples where debris have not been removed see Kiriaev et al. (2018). (A) A fiber containing a single branch with slits forming within the trunk. (B) A fiber showing rejoining of these slits within the trunk and multiple offshoots off the edge of the muscle. (C,D) Examples of complex branched muscle fibers.