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TPA treatment of cells does not prevent Ras binding to Ral-GDS. Cells were transfected as for Fig. 3 and treated with buffer or PMA. Myc-tagged Ral-GDS was then immunoprecipitated (IP) and immunoblotted for coprecipitated Ras61L. As a control, constitutively inactive Ras17N was transfected with Ral-GDS. The data are representative of two independent experiments. TPA, tetradecanoyl phorbol acetate.
