FIG. 5.

Deletion of the N terminus of Ral-GDS prevents PMA-induced suppression of Ral-GDS activation by Ras. (A) Map of Ral-GDS and an N-terminally truncated mutant, Ral-GDS(ΔN). CAT, catalytic domain; RBD, Ras binding domain. (B) Ral-GDS or Ral-GDS(ΔN) were transfected into COS-7 cells with GST-Ral or with GST-Ral plus constitutively activated Ras61L. Cells were treated with buffer or with PMA, and then the proportion of Ral in the GTP-bound state was measured as described for Fig. 3. The data represent the average of three independent experiments (± standard deviations).