Figure 1.

In vivo bioluminescence imaging (BLI) of engineered cells. (A) Cells are first genetically engineered to express the luciferase, then transplanted into the animal. The appropriate substrate is injected, and the emitted light is captured to generate an image. (B) Extinction coefficient value of water, oxyhemoglobin (HbO₂), and deoxyhemoglobin (Hb) for wavelengths in the visible to near-infrared region. Adapted with permission from Kobayashi et al.¹⁰ Copyright 2010 American Chemical Society.