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. 2021 Nov 15;16(12):2707–2718. doi: 10.1021/acschembio.1c00549

Figure 3.

Figure 3

Bioluminescence resonance energy transfer (BRET)-based luciferase systems: (A) A luciferase donor reacts with its substrate to produce light which is then absorbed by a fused fluorescent protein and re-emitted at a longer wavelength. (B) RLuc-based BRET systems and (C) NanoLuc-based BRET systems and their respective peak emission wavelengths.