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. 2021 Nov 29;11(24):14877–14883. doi: 10.1021/acscatal.1c03330

Figure 1.

Figure 1

NMT1 catalyzes N-terminal myristoylation and acetylation on ARF peptide. (A) Free and myristoylated ARF6 peptides are detected by HPLC and mass spectrometry. (B) Free and acetylated ARF6 peptides are detected by HPLC and mass spectrometry. (C) Plot of initial rates of reaction versus varying concentrations of myristoyl-CoA with ARF6 peptide concentrations at 1.6 (▲), 3.1 (▼), 6.3 (Δ), and 12.5 (▽) μM. (D) Plot of initial rates of reaction versus varying concentrations of acetyl-CoA with ARF6 peptide concentrations at 12.5 (▲), 25 (▼), 50 (Δ), and 100 (▽) μM. Data were globally fit with eq 2 (see Material and Methods in the Supporting Information), and the derived steady-state kinetic parameters are summarized in Table 1.