Table 2. Functional Analysis of the l-Lactate Biosensor in E. coli Nissle 1917a.
| condition | succinate
O2 (+) |
glucose
O2 (+) |
glucose
O2 (−) |
|||
|---|---|---|---|---|---|---|
| sensor | PLldPRD | ALPaGA | PLldPRD | ALPaGA | PLldPRD | ALPaGA |
| leakage RPU | 0.5 ± 0.0 | 1.0 ± 0.0 | 0.9 ± 0.3 | 1.4 ± 0.4 | 0.1 ± 0.0 | 0.5 ± 0.0 |
| max fold change | 7.8 ±0.5 | 11.8 ± 0.7 | 1.8 ± 1.3 | 8.1 ± 2.3 | 1.4 ± 0.4 | 5.1 ± 0.3 |
| max swing RPU | 6.4 ± 0.6 | 10.8 ± 0.3 | 0.8 ± 0.7 | 6.6 ± 1.4 | 0.06 ± 0.0 | 3.8 ± 0.8 |
| EC50(M) | 1.7 × 10–3 | 1.9 × 10–3 | 8.5 × 10–4 | 1.2 × 10–3 | ||
a
RPU: reference promoter units. The leakage RPU corresponds to the RPU in the non-induced state. The max fold change corresponds to the fold change between the induced and non-induced state. The max swing RPU corresponds to the subtraction of RPU between the induced and non-induced state. EC50 is the half-maximal effective concentration in molar. The equation was calculated by using the response function data from three experiments averaged (see Materials and Methods). (-: Unable to calculate).