Table 2. Growth Rate and Product Yields for Malate and Fumarate Relative to Biomass Either Predicted by FBA or Experimentally Observed during the Exponential Phase in Wild-Type Synechocystis and Derivative Strains Impaired in Malate Consumption Reactions^a2.

	growth rate (μ, h–1)		malate yield (Yp/x, mM gDW–1)		fumarate yield (Yp/x, mM gDW–1)
strains	model prediction	measured	model prediction	measured	model prediction	measured
wild type	0.052	0.055 ± 0.001	0	0	0	0
Δme	0.052	0.052 ± 0.000	0	0.067 ± 0.019	0	0.274 ± 0.014
Δmdh	0.052	0.055 ± 0.000	0	0	0	0
ΔmeΔmdh	0.050	0.050 ± 0.001	0–0.860	0.607 ± 0.091	0–0.860	0.557 ± 0.002

^aFVA was used for evaluating the robustness of the network and predicting the expected range for malate and/or fumarate production. The biomass equation was always used as the primary objective function in all FBA and FVA simulations. The experimental data are referred to the batch cultivation reported in Figure 2.