TABLE 2.
Intrachromosomal recombination in wild-type (Msh2+/+) and Msh2−/− cells
| Cell line | Frequency of neo+ recombinants (10−4)a
|
|
|---|---|---|
| DSB inducedb | Spontaneousc | |
| Wild-type clones | ||
| H-DR-WT | 1.8 ± 0.9 | 0.05 ± 0.05 |
| H-DR-8mu | 0.4 ± 0.1 | 0.004 ± 0.005 |
| Msh2−/− clones | ||
| H-DR-WT | 1.9 ± 0.5 | 0.08 ± 0.06 |
| H-DR-8mu | 1.6 ± 0.3 | 0.05 ± 0.02 |
a
Data are averages of four experiments with standard deviations, using two or three independently derived clones for each cell line.
b
Cells were transfected with the I-SceI expression vector, pPGK3xnlsI-SceI.
c
Cells were transfected with a control plasmid, pPGKlacZ.