FIG. 6.
GS and Ph activity in insulin-treated mice. (A and B) GS and Ph activity ratios were measured in skeletal muscle extracts as described in the legend to Fig. 3. The mice were treated with insulin (hatched bars) or not treated with insulin (open bars). Statistical significance was assessed by Student's t test (∗∗, P < 0.01). The number of animals analyzed is shown inside the bars. (C) Muscle extracts from control and insulin-treated mice were immunoblotted with anti-GS antibodies. (D) Muscle extracts from wild-type mice, control and insulin treated, were immunoblotted with antibodies that specifically recognize RGL phosphorylated at Ser48 (P-Ser48), RGL phosphorylated at Ser67 (P-Ser67), or nondiscriminating antibodies (RGL). Three representative independent samples per treatment are shown. +/+, wild type; −/−, RGL null mutant mice.