FIG. 7.
Insulin-stimulated glycogen accumulation and GSK-3, PTG, and R6 levels in wild-type and RGL knockout mice. (A) Glycogen content was measured as described in the legend to Fig. 4 in wild-type (+/+) and RGL homozygous knockout (−/−) mice with (hatched bars) and without (open bars) insulin treatment. The number of experiments is indicated inside the bars. Statistical significance was assessed by Student's t test (∗, P < 0.05). (B) Three representative samples from wild-type and RGL knockout mice were analyzed by immunoblotting with antibodies against GSK-3β, PTG, and R6. The numbers to the left show molecular size markers (in kilodattons).