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SC-MEB workflow. A. The SC-MEB workflow mainly comprises the following steps, data preprocessing, spatial clustering using the hidden MRF model, a series of downstream analyses. B. Data preprocessing: log-transformation, dimension reduction. C. The hidden MRF model. For the Visium dataset, we used six neighborhoods for each spot. D. The SC-MEB outputs: a scatter plot of MBIC for all K, a tissue plot with spots colored by clustering, a heatmap of DEGs.
