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. 2020 Dec 22;11(1):129–141. doi: 10.1039/d0ra07309a

Fig. 3. Cytotoxic and cytostatic effect of naringenin in HeLa cell spheroids. (A) Relative fluorescence units (RFU) of staining with Sytox Green® (dead cells) and Hoechst 33342 (total cell population) in HeLa cell spheroids exposed to 1, 4, and 7 days with 100, 300, and 500 μM of naringenin. (B) Epifluorescence microscopy images of spheroids treated with naringenin (100, 300, and 500 μM) during 1, 4, and 7 days stained with Sytox Green® (dead cells). p ≤ 0.05 according to Tukey's mean test, n = 12. Scale bar: 100 μm; amplification: 10×.

Fig. 3