Fig. 7. Effect of the combination of naringenin with cisplatin on the viability of HeLa cell spheroids at 24 h. (A) Decreased ATP levels of spheroids; (B) decreased viability and increased cytotoxicity in spheroids, through loss of membrane integrity, by staining with Sytox Green and Hoechst 33342, p ≤ 0.05 according to the Tukey's mean test, n = 12; (C) morphological changes in spheroids and fluorescence of Sytox Green indicating cell death. Scale bar: 100 μm; amplification: 10×.