FIG. 1.

Gal4-dependent activation is associated with deacetylation of histone H4. Cross-linked chromatin preparations from strain JDY7482 grown in the presence (+) or absence (−) of galactose were immunoprecipitated with antibodies against TBP (A) and acetylated (superscript Ac) H3 and H4 tails or unacetylated (superscript Unac) H4 tails (B). Immunoprecipitated and input material was analyzed by PCR with primers corresponding to the indicated promoters. Relative acetylation levels are indicated below each gel lane and were calculated as described in Materials and Methods.