FIG. 2.

Gcn4-dependent activation results in H3 and H4 hyperacetylation. Cross-linked chromatin preparations from strain JDY4251 grown in glucose minimal medium in the presence (+) or absence (−) of 10 mM aminotriazole were immunoprecipitated with antibodies against the acetylated tails of H3 and H4. Immunoprecipitated and input material was analyzed by PCR with primers corresponding to the indicated promoters. Relative acetylation levels are indicated below each gel lane and were calculated as described in Materials and Methods.