FIG. 3.

Transcriptional activation by Hap4 and Adr1 correlates with histone H4-specific deacetylation. Cross-linked chromatin preparations from cells grown in medium containing either glucose or ethanol as the sole carbon source were immunoprecipitated with antibodies against the acetylated (superscript Ac) tails of H3 and H4 or unacetylated (superscript Unac) H4 tails. Immunoprecipitated and input material was analyzed by PCR with primers corresponding to the indicated promoters. Relative acetylation levels are indicated below each gel lane and were calculated as described in Materials and Methods.