FIG. 7.

Genes repressed by Sin3-Rpd3 contain deacetylated histones H3 and H4 at their upstream regions. Chromatin extracted from formaldehyde-cross-linked cultures of strain JDY7841 and isogenic rpd3, sin3, and ume6 deletion strains was immunoprecipitated using antibodies against acetylated H3 and H4. (A) PCR products corresponding to the indicated promoters were generated from immunoprecipitated and input DNAs. Wt, wild type. (B) Rpd3-mediated deacetylation was mapped across the INO1 genomic locus using PCR primers centered around the indicated distances from the TATA region. The relative histone deacetylation caused by Rpd3 at each amplified region was calculated as described in Materials and Methods. A map of the INO1 locus indicates the position of the URS elements (black boxes), the UAS sequences (white boxes), and the TATA region (gray box).