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. 2021 Dec 20;19:521. doi: 10.1186/s12967-021-03178-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — SIRT5 desuccinylates PDHA1. A Flag-PDHA1 and HA-SIRT5 were transfected into HEK293T cells, and the pansuccinylation level of PDHA1 was determined. B HA-tagged PDHA1 and Flag-tagged SIRT5 were coexpressed in HEK293T cells. PDHA1 copurified with SIRT5 was detected using an HA antibody. C HA-tagged SIRT5 and Flag-tagged PDHA1 were coexpressed in HEK293T cells. SIRT5 copurified with PDHA1 was detected using an HA antibody. D Diluted succinyl-CoA was added to the purified PDHA1 system, and the pansuccinylation level of recombinant PDHA1 (rPDHA1) was determined. E The pBABE-SIRT5 and control vectors were introduced into HeLa cells, and pansuccinylation of PDHA1 was determined. F SIRT5 was knocked down in HeLa cells, and pansuccinylation of PDHA1 was determined