FIG. 7.

JNK antisense oligonucleotides attenuate T81 phosphorylation in vivo. MCF7 cells were transfected with either specific antisense oligonucleotides directed against JNK1 and JNK2 or nonspecific antisense oligonucleotides (250 nM each). Forty-eight hours after transfection, cells were either sham treated or UV treated and harvested 2 h after treatment. Top, specific T81 phosphorylation; middle, expression level of p53 in response to indicated treatments; bottom, expression of JNK1 and -2 in the presence or absence of transfected oligonucleotides. Control lane, no treatment; mock lipofection, cells maintained in concentrations of Lipofectin equal to those for the oligonucleotide-transfected samples; JNK1AS and JNK2AS, JNK antisense oligonucleotides that were transfected; JNKScr, control “scrambled” oligonucleotides.