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Induction of Cdk2-wt and Cdk2-dn in U2-OS clones. The designated clones were cultured in the presence (+) or absence (−) of Tet for 3 days. Protein extracts were subjected to immunoblotting with monoclonal antibody 12CA5 directed against the HA epitope tag (top) or a polyclonal antibody directed against Cdk2 (bottom).
