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Caffeine fails to rescue the S and G2/M arrests imposed by Cdk2-dn. Dn. 4 (A) and dn.2 (B) cells were incubated in the presence or absence of Tet for 48 h. A third culture maintained in Tet was subjected to 5 Gy of gamma irradiation (Irr) at the end of this time period. Each culture was then incubated in the presence or absence of 1 mM caffeine (Caf) for an additional 24 h and collected for flow cytometry. The percentage of cells in the G2/M fraction is given above each DNA profile.
