TABLE 1.
Caffeine does not mediate premature DNA condensation in cells with Cdk2-dn inductiona
Transfection | Condensed nuclei (%)b
|
|
---|---|---|
+Tet | −Tet | |
No caffeine | 5.3 ± 0.9 | 3.2 ± 2.6 |
Caffeine | 20 ± 2.0 | 5.1 ± 2.4 |
Cells were synchronized with HU, released for 4 h, incubated with 1 mM caffeine for another 8 h, fixed, and stained with bisbenzimide (Hoechst).
Mean ± standard deviation from three independent experiments.