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Transfection of Cdk2-wt rescues the inhibition of DNA condensation mediated by Cdk2-dn inductiona
| Transfection | Condensed nuclei (%)b
|
|
|---|---|---|
| +Tet | −Tet | |
| Vector | 66 ± 6 | 40 ± 10 |
| Cdk2-wt | 73 ± 2 | 65 ± 8 |
Cells were transfected with vector or Cdk2-wt and incubated in medium with or without Tet. The cells were then synchronized with HU, released in the presence of nocodazole for 18 h, fixed, and stained with bisbenzimide.
Mean ± standard deviations from three independent experiments.
