Table 1.
Primary and secondary antibodies
| Primary antibodies | ||||||
|---|---|---|---|---|---|---|
| RRID | Antigen | Host | Dilution | Supplier | Catalog number | |
| AB_1658411 | CGRP | Mouse | 1:500 | Abcam | AB81887 | |
| AB_11214092 | ChAT | Goat | 1:500 | Millipore | AB144P | |
| AB_258833 | CTB | Rabbit | 1:30,000 (sections); 1:3000 (iDisco clearing) |
Sigma-Aldrich | C3062 | |
| AB_477257 | NF200 | Mouse | 1:4000 | Sigma-Aldrich | NO142 | |
| AB_90755 | TH | Sheep | 1:1000 | Millipore | AB1542 | |
| AB_1624144 | TRPV1 | Goat | 1:1000 | Neuromics | GT15129 | |
| Secondary antibodies | ||||||
| RRID | Antigen | Tag | Host | Dilution | Supplier | Catalog number |
| AB_2307351 | Goat IgG | Cy3 | Donkey | 1:2000 | Jackson ImmunoResearch | 705-165-147 |
| AB_2340433 | Goat IgG | AF594 | Donkey | 1:500 | Jackson ImmunoResearch | 705-585-147 |
| AB_2340846 | Mouse IgG | AF488 | Donkey | 1:2000 | Jackson ImmunoResearch | 711-545-150 |
| AB_2535789 | Mouse IgG | AF594 | Donkey | 1:1000 | Molecular Probes | A-21203 |
| AB_2313584 | Rabbit IgG | AF488 | Donkey | 1:1000 | Jackson ImmunoResearch | 711-545-152 |
| AB_2307443 | Rabbit IgG | Cy3 | Donkey | 1:3000 | Jackson ImmunoResearch | 711-165-152 |
| AB_2536183 | Rabbit IgG | AF647 | Donkey | 1:1000 | Molecular Probes | A-31573 |
| AB_10374882 | Sheep IgG | AF647 | Donkey | 1:500 | Molecular Probes | A-21448 |
CGRP, calcitonin gene-related peptide; ChAT, choline acetyltransferase; CTB, cholera toxin B subunit; NF200, neurofilament 200 kDa; TH, tyrosine hydroxylase; TRPV1, transient receptor potential vanilloid 1.