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. 2022 Jan 1;12(1):167–185. doi: 10.7150/thno.61209

Figure 2.

Figure 2

Memory CD4 and CD8 TILs in MPM overexpress PD1 while downregulated CD127. (A) Representative MPM specimens showing differences in distribution of TILs in serial histological sections (scale bar: 50 µm; H&E, CD8, CD4). (B-D) Bivariate plots showing gating strategy to select single live T cells (B) and co-expression of PD1, CD107a and CD127 in CD4 (C) and CD8 (D) memory compartments. (E-F) Scatter plots showing the frequency of CD4 and CD8 TILs (E) and CD4/CD8 ratio (F). (G-H) Scatter plots showing the frequency of CD4+ (top) and CD8+ TILs (bottom) subgated for PD1 and CD107a. (I) Scatter plots of CD4 (top) and CD8 memory TILs (bottom) showing geometric mean fluorescence intensity (gMFI) of CD127 in cell subsets subgated based on PD1 expression in MPM. All data in E-I determined by flow cytometry. Significant differences in E-H calculated between matched tumor (T) and non-adjacent lung control tissue pairs (N) in 9 patients using Wilcoxon signed-rank test. All tests were two-tailed. See also Figure S2 in the online data supplement for full gating strategy.