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. 2022 Jan 1;12(1):379–395. doi: 10.7150/thno.67034

Figure 9.

Figure 9

Silencing E2F3 abolishes the augmented β cell proliferation in cultured Smad3KO islet cells. (A) Dissociated Smad3WT islet cells were cultured in the presence of Ad-shE2F3 or Ad-shCon virus for 48 h. E2F3 expression was detected by Western blot. n = 3 culture replicates in each group. **p < 0.01 compared with Ad.shCon. (B, C) Dissociated Smad3WT and Smad3KO islet cells were cultured in the presence of Ad-shE2F3 or Ad-shCon virus for 48 h followed by BrdU labeling for another 24 h. β cell proliferation was shown by double immunostaining for insulin+ BrdU+ cells (red nuclei). Each dot represents one independent experiment and data are expressed as mean ± SD. Scale bar, 100 μm. **p < 0.01, ***p < 0.001 versus Smad3WT shCon. ###p < 0.001 versus Smad3KO Ad.shCon.