Figure 7.

Nanoparticle-loaded single-stranded circDNA served as a miR-9 sponge that increased tumor suppressor gene expression and inhibited tumor proliferation and metastasis. (A) Motifs from miRNAs targeting KLF17, CDH1, and LASS2 found by a database search. (B) Several miR-9 sponges (linear RNA-9, circR-9, CSSD-9-s, and CSSD-9) were designed. (C) qRT-PCR detection of miR-9 expression in HeLa cells after transfection of nanoparticles with different miR-9 sponges for different time points. miR-9 expression was normalized by reference gene U6 small nuclear RNA expression. (D) Western blot analysis of KLF17, CDH1, and LASS2 proteins in HeLa cells transfected with nanoparticles with different miR-9 sponges. Reprinted from ⁶⁶, copyright (2018) The Science Publishing Group.