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Total RNA from an expanded clonal population of GFP-expressing cells stably transfected with U1(H) snRNA and U1(H) anti-GFP derived from the experiment shown in Fig. 4C. Clone C, U1(H) snRNA; clone F, U1(H) anti-GFP. GFP mRNA was normalized to GAPDH. U6 snRNA was used to show the efficiency of nuclear and cytoplasmic RNA extraction. The lower panel shows the ethidium bromide-stained agarose gel.
