TABLE 2.
Fluorescence of a pooled population of GFP-expressing cells as determined by flow cytometry
| Sample | Description | % in M2 windowa | Mean intensity | Fluorescence index |
|---|---|---|---|---|
| 1 | Control (nontransfected) | 0.16 | 333 | 52 |
| 2 | Parental cells (nontransfected) | 94.5 | 1,804 | 171,000 |
| 3 | Parental + U1(H) snRNA | 92.5 | 1,873 | 170,000 |
| 4 | Parental + U1(H) antiGFP | 60.5 | 851 | 51,000 |
M2 is the window in the FACS scan where the fluorescent signal exceeds the background of GFP negative cells. (See Fig. 4B.)