TABLE 3.
Fluorescence of an isolated clonal population of GFP-expressing cells as determined by flow cytometry
| Sample | Description | % in M2 windowa | Mean intensity | Fluorescence index |
|---|---|---|---|---|
| C | Parental + U1(H) snRNA | 89.6 | 1,014 | 90,900 |
| D | Parental + U1(H) snRNA | 92.0 | 1,119 | 102,900 |
| E | Parental + U1(H) antiGFP | 57.5 | 200 | 11,500 |
| F | Parental + U1(H) antiGFP | 51.6 | 183 | 9,450 |
| G | Parental + U1(H) antiGFP | 53.5 | 180 | 9,640 |
| H | Parental + U1(H) antiGFP | 86.8 | 954 | 82,800 |
a
M2 is the window in the FACS scan where the fluorescent signal exceeds the background of GFP negative cells (see Fig. 4C).