Comparative effect of diallyl trisulfide (DATS) on cytokine expression in TNF-alpha induced MDA-MB-231 and MDA-MB-468 breast cancer cells (n=3). The array layout used to assess chemokines/cytokines expression in supernatants of treated cells, showing the cytokines map, and highlighting CCL2/MCP-1, IL-6, PDGF-BB, NT-3, and positive controls. (A) (C) supernatant of cells treated with TNF-α and (B) (D) supernatant of cells co-treated with TNF-α and DATS Chemiluminescent spot intensity derived indicates changes in cytokine expression after 24 h.