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. Author manuscript; available in PMC: 2021 Dec 21.

Published in final edited form as: Anticancer Res. 2021 Dec;41(12):5919–5933. doi: 10.21873/anticanres.15411

Figure 6. — Quantification of mRNA expression in MDA-MB-231 and MDA-MB-468 triple negative breast cancer cells using RT-PCR. MDA-MB-231 and MDA-MB-468 TNBC cells were treated with diallyl trisulfide (DATS) (75 μM), TNF-α (40 ng/ml), the combination of DATS (75 μM)+TNF-α (40 ng/ml) or left untreated. The normalized levels of CCL2, IKBKE, and MAPK8 mRNA were assayed by using RT-PCR. Each data point represents the mean±S.E.M. of three independent experiments (n=3). Statistically significant differences between TNF-α vs. treatments were evaluated by a one-way ANOVA, followed by Dunnett’s multiple comparison test with *p<0.05, **p<0.01, and ***p<0.001.

Figure 6. — Quantification of mRNA expression in MDA-MB-231 and MDA-MB-468 triple negative breast cancer cells using RT-PCR. MDA-MB-231 and MDA-MB-468 TNBC cells were treated with diallyl trisulfide (DATS) (75 μM), TNF-α (40 ng/ml), the combination of DATS (75 μM)+TNF-α (40 ng/ml) or left untreated. The normalized levels of CCL2, IKBKE, and MAPK8 mRNA were assayed by using RT-PCR. Each data point represents the mean±S.E.M. of three independent experiments (n=3). Statistically significant differences between TNF-α vs. treatments were evaluated by a one-way ANOVA, followed by Dunnett’s multiple comparison test with *p<0.05, **p<0.01, and ***p<0.001.