TABLE 1.
Results after three rounds of screening of pDF-lib for IRES elementsa
| Sequence name | No. of times identified | Sequence |
|---|---|---|
| PS1 | 5 | 5′ CACAgTACgTAAgCTTAAgCTAAgCgTAgATAAgggTATATTTTTgCg 3′ |
| PS2 | 21 | 5′ gAAATAgCTATCCTCCATCACTgCACCgAgACTACggTTgCgCgTgTCgT 3′ |
| PS3 | 52 | 5′ TgACAAACTgTACATgCCgTTAACTgTAATTTTgCgTgATTTTTTTgTAg 3′ |
| PS4 | 7 | 5′ AggTggTAgCCgCAAACATAgTTCAATACAAACTTgCTgTCTCggCgg 3′ |
| PS5 | 12 | 5′ AggCAgTATAATCAgTTCCCACATAgAAAACCAggACTgTATCAAAgTgT 3′ |
a
After three rounds of selection, 158 individual clones were assayed by transfection. Of these, 97 were identified as a strong IRES element (i.e., expressed a significant level of EGFP). Five different sequences were found.