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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Cell Signal. 2020 Nov 24;78:109847. doi: 10.1016/j.cellsig.2020.109847

Fig. 1. DA inhibits OC differentiation from RAW cells.

Fig. 1.

RAW cells were treated with indicated concentration of DA in the presence of 10 ng/mL RANKL for 4 days. (A) TRAP staining and quantification of OC. TRAP positive, multinucleated OC in red were counted. n=4. Scale bar is 200μm. (B) Relative expression of osteoclastic genes (c-Fos, Nfatc1, Trap, Ctsk) on day 4 after DA+RANKL treatment were determined by RT-qPCR; normalized to B2m. n=3. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl group. Data shown as mean ± SEM.