Figure 3. CDKA1 regulates cell size dependence of commitment.

Photoautotrophic cultures of WT and cdka1 were plated directly from light (A) or after 8.5 hrs dark incubation (B). Plates received the indicated periods of light before transfer to dark. Images were taken at plating, dark transfer and 30 hrs after plating. ‘Unco’ (uncommitted): no division at 30 hrs; ‘Co’ (committed): no division at time of dark transfer, but division at 30 hrs; ‘Div’ (divided): divided at time of dark transfer. C. Cell sizes at dark transfer were determined by automated segmentation, and cells sorted into 0.25-log2 size bins. Proportions of binned cells that divided after dark incubation was determined (pooled data for two strains of each genotype).