FIG. 7.

Nucleosome arrays associated with genes active in heterochromatin compared to the euchromatic transgene. Blots containing DNA samples from nuclei digested with MNase as described in the legend to Fig. 2 were hybridized with a 2.4-kb BamHI-PstI fragment encompassing most of the first exon and ca. 1 kb of upstream DNA at light (20) (B), a 1.4-kb rolled gene cDNA (C), a 1,463-bp fragment starting at position +106 and extending upstream, encompassing the putative 5′ regulatory region of rolled (D), and a 813-bp fragment located 14038 bp upstream of the rolled gene start site (E). The fragments used for panels D and E were prepared by PCR from NCBI clone AE003090. Panel A shows the nucleosome array of the transgene in a euchromatic site (line 39C-X) for reference.