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. 2021 Dec 7;8:780865. doi: 10.3389/fmolb.2021.780865

FIGURE 1.

FIGURE 1

Effects of NAD+ on O-GlcNAc levels and ER stress in response to glucose deprivation in AC16 cells. (A) Left panel: O-GlcNAc, OGA and OGT immunoblots, with and without glucose (5 mM) and NAD+ (250 µM). Right panel: Quantification of immunoblots normalized to GAPDH. (B) Left panel: O-GlcNAc and acetylated lysine immunoblots, with and without glucose (5 mM) and nicotinamide (NAM, 5 mM). Right panel: Quantification of immunoblots normalized to GAPDH. (C) Left panel: Effects of SIRT1 inhibitor EX527 (10 µM) on O-GlcNAc and acetylated lysine levels in response to glucose deprivation with NAD+. Right panel: Quantification of immunoblots normalized to GAPDH. (D) Left panel: BiP, CHOP, phospho- (P) and total (T) PERK immunoblots, with and without glucose (5 mM) and NAD+ (250 µM). Right panel: Quantification of immunoblots normalized to GAPDH. *p < 0.05 vs. Control (Con) group; #p < 0.05 vs. glucose deprivation (GD) group. All data are expressed as mean ± S.E.M. of 3–6 independent experiments.