FIG. 3.

Analysis of deletion mutants of Gallus BSP promoter in ROS 17/2.8 osteoblastic cells. (A) Schematic illustration of 5′ BSP promoter deletion constructs used in transient transfection assays. Col II-labeled octagons, silencer motifs; VDRE, vitamin D response element. (B) Assay of BSP promoter-CAT reporter constructs in ROS 17/2.8 cells. Cells were transfected with the indicated reporter constructs. Reporter activity was assayed 24 h after transfection and normalized for transfection efficiency (see Materials and Methods). Data are means + the standard errors of the means (SEM), (n = 18 samples); data from three independent experiments were pooled and represent multiple DNA preparations.