| Strain, strain background (Mus musculus) |
NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ |
https://www.jax.org/strain/005557
|
MGI:3577020 |
8–12 weeks old |
| Sequence-based reagent |
Human RT-PCR Primers |
Applied Biosystems Taqman Assays |
ACTBHs01060665_g1 |
|
| Sequence-based reagent |
Human RT-PCR Primers |
Applied Biosystems Taqman Assays |
DYSTROPHINHs007758098_m1 |
|
| Sequence-based reagent |
Human RT-PCR Primers |
Applied Biosystems Taqman Assays |
PAX7Hs00242962_m1 |
|
| Sequence-based reagent |
Human RT-PCR Primers |
Applied Biosystems Taqman Assays |
CD56Hs00941830_m1 |
|
| Sequence-based reagent |
Human RT-PCR Primers |
Applied Biosystems Taqman Assays |
MYOD1Hs00159528_m1 |
|
| Sequence-based reagent |
Human RT-PCR Primers |
Applied Biosystems Taqman Assays |
MYF5Hs00929416_g1 |
|
| Sequence-based reagent |
Human RT-PCR Primers |
Applied Biosystems Taqman Assays |
MYOGENIN Hs01072232_m1 |
|
| Sequence-based reagent |
Human RT-PCR Primers |
Applied Biosystems Taqman Assays |
COL1A1Hs01076780_g1 |
|
| Sequence-based reagent |
Human RT-PCR Primers |
Applied Biosystems Taqman Assays |
ID1Hs03676575_s1 |
|
| Sequence-based reagent |
Human RT-PCR Primers |
Applied Biosystems Taqman Assays |
ID3Hs00954037_g1 |
|
| Antibody |
Anti-Human DYSTROPHIN (mouse monoclonal) |
DSHB |
AB_2618157
|
IF(1:10) |
| Antibody |
Anti-Human/Mouse DYSTROPHIN (mouse monoclonal) |
Thermofisher |
AB_10978300
|
IF(1:500) |
| Antibody |
Anti-Human/Mouse PAX7 (mouse monoclonal) |
DSHB |
AB_528428
|
IF(1:10) |
| Antibody |
Anti-Human/Mouse MHC (mouse monoclonal) |
DSHB |
AB_2147781
|
IF(1:100) |
| Antibody |
Anti-LAMININ (rabbit polyclonal) |
Sigma-Aldrich |
AB_477163
|
IF(1:250) |
| Antibody |
Anti-Human SPECTRIN (mouse monoclonal) |
Leica Microsystems |
AB_442135
|
IF(1:100) |
| Antibody |
Anti-Human LAMIN A/C (mouse monoclonal) |
Vector Laboratories |
AB_2336546
|
IF(1:100) |
| Antibody |
Anti-Human CD31 (Beads) (Mouse monoclonal) |
Miltenyi Biotec |
130-091-935 |
2 μl |
| Antibody |
Anti-Human CD45 (Beads, mouse monoclonal) |
Miltenyi Biotec |
AB_2783001
|
5 μl |
| Antibody |
Anti-Human CD31 AF450(WM-59, mouse monoclonal) |
Ebioscience |
AB_10854276
|
5 μl |
| Antibody |
Anti-Human CD34 eFluor450(4H11, mouse monoclonal) |
Ebioscience |
AB_10733282
|
5 μl |
| Antibody |
Anti-Human CD45 AF450(30-F11, mouse monoclonal) |
Ebioscience |
AB_1518806
|
5 μl |
| Antibody |
Anti-Human CD29 FITC(TS2/16, mouse monoclonal) |
Ebioscience |
AB_2043830
|
8 μl |
| Antibody |
Recombinant human anti-CD56 APC-vio-770 (REA196) |
Miltenyi Biotec |
AB_2733136
|
8 μl |
| Antibody |
Anti-Human CXCR4 PE(12G5, mouse monoclonal) |
Ebioscience |
AB_10669164
|
8 μl |
| Antibody |
Anti-Human HNK1 PE (TB01, mouse monoclonal) |
Ebioscience |
AB_10804531
|
5 μl |
| Antibody |
Anti-Human CD45 PE(30-F11, mouse monoclonal) |
Ebioscience |
AB_465668
|
5 μl |
| Antibody |
Anti-Human CD31 PE(390, mouse monoclonal) |
Ebioscience |
AB_465632
|
5 μl |
| Antibody |
Anti-Human CD56 APC (CMSSB, mouse monoclonal) |
Ebioscience |
AB_10854573
|
5 μl |
| Antibody |
Anti-Human CXCR4 PE-Cy7 (12G5, mouse monoclonal) |
Ebioscience |
AB_1659706
|
5 μl |
| Antibody |
FcR block |
Miltenyi Biotec |
AB_2892112
|
5 μl |
| Software, algorithm |
GraphPad Prism |
GraphPad Prism (https://graphpad.com) |
SCr_002798
|
|
| Software, algorithm |
Seurat (3.1.5) |
https://satijalab.org/seurat/
|
SCR_007322
|
SCR_021002
|
| Software, algorithm |
Monocle (2.12.0) |
http://cole-trapnell-lab.github.io/monocle-release/
|
SCR_016339
|
|
| Software, algorithm |
cellranger |
https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/feature-bc
|
SCR_021002
|
|
| Software, algorithm |
FlowJo |
https://www.flowjo.com
|
SCR_008520
|
|
