Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Commercial assay or kit |
KAPA-HiFi HotStart ReadyMix |
Roche | Material # 7958935001 | |
| Commercial assay or kit |
NEBuilder HiFi DNA Assembly Master Mix |
NEB | Cat # E2621L | |
| Commercial assay or kit |
In-Fusion HD Cloning Plus Kits |
Takara | Cat # 638,911 | |
| Recombinant DNA reagent |
pSA293- CHEQ-seq |
This paper | Addgene: 174669;RRID:Addgene_174669 | CHEQ-seq backbone vector |
| Other | MF-Millipore 0.05 µm |
Merck | Ref: VMWP02500 | 25 mm diameter, mixed cellulose esters (MCE) membrane |
| Strain, strain background (Escherichia coli) |
Endura Electro Competent Cells |
Lucigen | Cat # 60242-2 | Electrocompetent bacteria |
| Commercial assay or kit |
MinElute PCR Purification Kit | QIAGEN | ID: 28004 | |
| Commercial assay or kit |
SPRIselect | Beckman Coulter | Product # B23317 | |
| Commercial assay or kit | innuPREP RNA Mini Kit 2.0 |
Analytik Jena | Cat # 845-KS-2040250 | |
| Commercial assay or kit | Dynabeads mRNA purification kit |
Ambion | Cat # 61006 | |
| Commercial assay or kit |
GoScript RT kit | Promega | Cat # A5003 | |
| Recombinant DNA reagent |
pGL4.23-GW | Promega | Addgene: 60323;RRID:Addgene_60323 | Luciferase backbone vector |
| Recombinant DNA reagent |
pRL-TK | Promega | Cat # E2241 | Renilla vector |
| Commercial assay or kit |
Dual-Luciferase Reporter Assay System |
Promega | Cat # E1910 |