| Antibody |
Anti-mouse CD3-eFluor 450 (clone: 17A2; rat monoclonal) |
eBioscience |
Cat#: 48-0032-82; RRID:AB_1272193
|
Flow Cytometry: (dilution 1:30) |
| Antibody |
Anti-mouse CD8a-PerCP-Cyanine5.5 (clone: 53–6.7; rat monoclonal) |
eBioscience |
Cat#: 45-0081-82; RRID:AB_1107004
|
Flow Cytometry: (dilution 1:100) |
| Antibody |
Anti-mouse CD11b-Alexa Fluor 700 (clone: M1/70; rat monoclonal) |
eBioscience |
Cat#: 56-0112-82; RRID:AB_1107004
|
Flow Cytometry: (dilution 1:300) |
| Antibody |
Anti-mouse CD11b-eFluor450 (clone: M1/70; rat monoclonal) |
eBioscience |
Cat#: 48-0112-82; RRID:AB_1582236
|
Flow Cytometry: (dilution 1:500) |
| Antibody |
Anti-mouse CD14-FITC (clone: Sa2-8; rat monoclonal) |
eBioscience |
Cat#: 11-0141-85; RRID:AB_464950
|
Flow Cytometry: (dilution 1:20) |
| Antibody |
Anti-mouse CD25-APC (clone: PC61.5; rat monoclonal) |
eBioscience |
Cat#: 17-0251-82; RRID:AB_469366
|
Flow Cytometry: (dilution 1:500) |
| Antibody |
Anti-mouse CD25-eFluor 450 (clone: PC61.5; rat monoclonal) |
eBioscience |
Cat#: 48-0251-82; RRID:AB_10671550
|
Flow Cytometry: (dilution 1:400) |
| Antibody |
Anti-mouse CD45.1-APC (clone: A20; mouse monoclonal) |
eBioscience |
Cat#: 17-0453-82; RRID:AB_469398
|
Flow Cytometry: (dilution 1:200) |
| Antibody |
Anti-mouse Ly-6C-APC-eFluor 780 (clone: HK1.4; rat monoclonal) |
eBioscience |
Cat#: 47-5932-82; RRID:AB_2573992
|
Flow Cytometry: (dilution 1:200) |
| Antibody |
Anti-mouse MHC Class II (I-A/I-E)-FITC (clone: M5/114.15.2; rat monoclonal) |
eBioscience |
Cat#: 11-5321-82; RRID:AB_465232
|
Flow Cytometry: (dilution 1:300) |
| Antibody |
Anti-mouse CD45.1-eFluor 450 (clone: A20; mouse monoclonal) |
eBioscience |
Cat#: 48-0453-82; RRID:AB_1272189
|
Flow Cytometry: (dilution 1:200) |
| Antibody |
Anti-mouse CD45/B220-Horizont V500 (clone: RA3-6B2; rat monoclonal) |
BD Biosciences |
Cat#: 561226; RRID:AB_10563910
|
Flow Cytometry: (dilution 1:100) |
| Antibody |
Anti-mouse CD3e-Horizont V500 (clone: 500A2; Syrian hamster monoclonal) |
BD Biosciences |
Cat#: 560771; RRID:AB_1937314
|
Flow Cytometry: (dilution 1:30) |
| Antibody |
Anti-mouse CD4-Horizont V500 (clone: RM4.5; rat monoclonal) |
BD Biosciences |
Cat#: 560782; RRID:AB_1937327
|
Flow Cytometry: (dilution 1:400) |
| Antibody |
Anti-mouse CD4-PerCP (clone: RM4-5; rat monoclonal) |
BD Biosciences |
Cat#: 553052; RRID:AB_394587
|
Flow Cytometry: (dilution 1:200) |
| Antibody |
Anti-mouse CD8a-V500 (clone: 53–6.7; rat monoclonal) |
BD Biosciences |
Cat#: 560776; RRID:AB_1937317
|
Flow Cytometry: (dilution 1:80) |
| Antibody |
Anti-mouse Ly-6G-Alexa Fluor 700 (clone: 1A8; rat monoclonal) |
BD Biosciences |
Cat#: 561236; RRID:AB_10611860
|
Flow Cytometry: (dilution 1:80) |
| Antibody |
Anti-mouse Siglec-F-PE (clone: E50-2440; rat monoclonal) |
BD Biosciences |
Cat#: 562068; RRID:AB_394341
|
Flow Cytometry: (dilution 1:100) |
| Antibody |
Anti-mouse TLR4-APC (clone: SA15-21; rat monoclonal) |
BioLegend |
Cat#: 145406; RRID:AB_2562503
|
Flow Cytometry: (dilution 1:300) |
| Antibody |
Anti-mouse TNF alpha-PE (clone: MP6-XT22; rat monoclonal) |
eBioscience |
Cat#: 12-7321-82; RRID:AB_466199
|
Flow Cytometry: (dilution 0.35 µL/test) |
| Antibody |
Anti-FOXP3-PE (clone: FJK/16 s; rat monoclonal) |
eBioscience |
Cat#: 12-5773-82; RRID:AB_465936
|
Flow Cytometry: (dilution 1 µL/test) |
| Antibody |
Anti-mouse IFN gamma-PE (clone: XMG1.2; rat monoclonal) |
eBioscience |
Cat#: 12-7311-82; RRID:AB_466193
|
Flow Cytometry: (dilution 0.35 µL/test) |
| Antibody |
Anti-mouse CD16/32 (clone: 03; rat monoclonal) |
eBioscience |
Cat#: 14-0161-86; RRID:AB_467135
|
Flow Cytometry: (dilution 0.5 µg/test) |
| Antibody |
Anti-mouse IFN gamma (clone: XMG1.2; rat monoclonal) |
BioXCell |
Cat#: BE0055; RRID:AB_1107694
|
In vivo IFN gamma neutralization (dose: 250 µg/mouse) |
| Antibody |
Anti-mouse IL-2 (clone: JES6-1A12; rat monoclonal) |
BioXCell |
Cat#: BE0043; RRID:AB_1107702
|
In vivo IL-2 receptor stimulation as a complex with IL-2 (dose: 1.5 µg /mouse) |
| Antibody |
Anti-mouse IL-2 (clone: S4B6-1; rat monoclonal) |
BioXCell |
Cat#: BE0043-1; RRID:AB_1107705
|
In vivo IL-2 receptor stimulation as a complex with IL-2 (dose: 1.5 µg /mouse) |
| Antibody |
Anti-mouse CD4 (clone: GK1.5; rat monoclonal) |
BioXCell |
Cat#: BE0003-1; RRID:AB_1107636
|
In vivo CD4+ T cell depletion (dose: 50 µg /mouse) |
| Antibody |
Anti-mouse CD25 (clone: PC61.5; rat monoclonal) |
BioXCell |
Cat#: BE0012; RRID:AB_1107619
|
In vivo regulatory T cell depletion (dose: 50 µg /mouse) |
| Sequence-based Reagent |
Casc3for |
Sigma-Aldrich |
|
5´-TTCGAGGTGTGCCTAACCA-3´ |
| Sequence-based Reagent |
Casc3rev |
Sigma-Aldrich |
|
5´-GCTTAGCTCGACCACTCTGG-3´ |
| Sequence-based Reagent |
H6pdfor |
Sigma-Aldrich |
|
5´-GGATTGTGTTTAAGAATCGGG-3´ |
| Sequence-based Reagent |
H6pdrev |
Sigma-Aldrich |
|
5´-AGTAGGCGTCTTGCTC-3´ |
| Sequence-based Reagent |
Tlr4for |
Sigma-Aldrich |
|
5´-GATCATGGCACTGTTCTTCTC-3´ |
| Sequence-based Reagent |
Tlr4rev |
Sigma-Aldrich |
|
5´-CACACCTGGATAAATCCAGC-3´ |
| Strain, Strain Background (Salmonella Typhymurium) |
LT2 (S-strain) |
Gift from Dr. H. Nikaido (University of California, Berkeley) |
|
LPS isolation |
| Strain, Strain Background (Mus musculus; Female) |
BALB/c (H-2d) |
Institute of Microbiology and Institute of Physiology of the Czech Academy of Sciences |
|
|
| Strain, Strain Background (Mus musculus; Male) |
C57BL/6 (H-2b) |
Institute of Microbiology and Institute of Physiology of the Czech Academy of Sciences |
|
|
| Strain, Strain Background (Mus musculus; Female) |
CD1 nude mice (Nu/Nu) |
Animal facility of Masaryk University, Czech Republic |
|
|
| Strain, Strain Background (Mus musculus, C57BL/6 J) |
Transgenic OVA-specific T cells (OT-I) mice |
Institute of Microbiology of the Czech Academy of Sciences |
|
|
| Strain, Strain Background (Mus musculus, C57BL/6 J) |
Transgenic OVA-specific T cells (OT-II) mice |
Institute of Microbiology of the Czech Academy of Sciences |
|
|
| Strain, Strain Background (Mus musculus; C57BL/6 J) |
IFN gamma deficient (Ifng-/-) |
Institute of Microbiology of the Czech Academy of Sciences |
|
|
| Strain, Strain Background (Mus musculus; C57BL/6 J) |
MyD88 deficient (Myd88-/-) |
Institute of Molecular Genetics of the Czech Academy of Sciences |
|
|
| Strain, Strain Background (Mus musculus; C57BL/6 J) |
Rag1 deficient (Rag1-/-) |
Institute of Molecular Genetics of the Czech Academy of Sciences |
|
|
| Strain, Strain Background (Mus musculus, C57BL/6 J) |
B6.SJL (Ly5.1) |
Institute of Microbiology of the Czech Academy of Sciences |
|
|
| Peptide, Recombinant Protein |
Recombinant Murine IL-2 |
PeproTech |
Cat#: 212–12 |
Purified from E. coli
|
| Commercial Assay or Kit |
Mouse TNF-alpha DuoSet ELISA |
R&D Systems |
Cat#: DY410 |
|
| Commercial Assay or Kit |
Mouse IFN-gamma DuoSet ELISA |
R&D Systems |
Cat#: DY485 |
|
| Commercial Assay or Kit |
Mouse IL-1 beta/IL-1F2 DuoSet ELISA |
R&D Systems |
Cat#: DY401 |
|
| Commercial Assay or Kit |
Mouse IL-12 p70 DuoSet ELISA |
R&D Systems |
Cat#: DY419 |
|
| Commercial Assay or Kit |
Mouse IL-6 DuoSet ELISA |
R&D Systems |
Cat#: DY406 |
|
| Chemical Compound, Drug |
Brefeldin A |
Sigma-Aldrich |
Cat#: B7651 |
150 µg/mouse |
| Software, Algorithm |
FlowJo |
Tree Star |
RRID: SCR_008520
|
|
| Software, Algorithm |
GraphPad Prism |
GraphPad Software |
RRID: SCR_002798
|
|
| Other |
ACK Lysing Buffer |
Thermo Fisher Scientific |
Cat#: A1049201 |
Red blood cell lysis |
| Other |
Collagenase D |
Sigma-Aldrich (Roche) |
Cat#: 11088858001 |
1 mg/mL |
| Other |
Foxp3/ Transcription Factor Fixation/Permeabilization Concentrate and Diluent |
eBioscience |
Cat#: 00-5521-00 |
|
| Other |
Polyinosinic-polycytidylic acid potassium salt (Poly I:C) |
Sigma-Aldrich |
Cat#: P9582 |
75 µg/mouse |
| Other |
5-Bromo-2′-deoxyuridine (BrdU) |
Sigma-Aldrich (Roche) |
Cat#: 10280879001 |
0.5 µg/mouse i.p., 0.8 mg/mL p.o. |
| Other |
TRIzol Reagent |
Thermo Fisher Scientific (Invitrogen) |
Cat#: 15596026 |
|
| Other |
TURBO DNase |
Thermo Fisher Scientific |
Cat#: AM2238 |
|
| Other |
SuperScript IV Reverse Transcriptase |
Thermo Fisher Scientific |
Cat#: 18090010 |
|
