| Strain, strain background (Oryzias latipes) |
Cab |
Other |
|
Medaka Southern wild-type population |
| Strain, strain background (Oryzias latipes) |
eGFP-cbx1b
|
This paper |
|
CRISPR KI line, Aulehla Lab EMBL Heidelberg |
| Strain, strain background (Oryzias latipes) |
mGL-cbx1b
|
This paper |
|
CRISPR KI line, Aulehla Lab EMBL Heidelberg |
| Strain, strain background (Oryzias latipes) |
cdh2-eGFP
|
This paper |
|
CRISPR KI line, Aulehla Lab EMBL Heidelberg |
| Strain, strain background (Oryzias latipes) |
g3bp1-eGFP
|
This paper |
|
CRISPR KI line, Aulehla Lab EMBL Heidelberg |
| Strain, strain background (Oryzias latipes) |
mapre1b-linker-3XFlag-mScarlet
|
This paper |
|
CRISPR KI line, Aulehla Lab EMBL Heidelberg |
| Strain, strain background (Oryzias latipes) |
mNG-HAtag-Linker-myosinhc
|
This paper |
|
CRISPR KI line, Aulehla Lab EMBL Heidelberg |
| Strain, strain background (Oryzias latipes) |
mScarlet-pcna
|
This paper |
|
CRISPR KI line, Aulehla Lab EMBL Heidelberg |
| Strain, strain background (Oryzias latipes) |
eGFP-rab11a
|
This paper |
|
CRISPR KI line, Aulehla Lab EMBL Heidelberg |
| Strain, strain background (Oryzias latipes) |
(eGFP-cbx1b) × (mScarlet-pcna) |
This paper |
|
CRISPR KI line, Aulehla Lab EMBL Heidelberg |
| Antibody |
Primary rabbit anti-GFP (polyclonal) |
TorreyPines Biolabs |
#TP401 |
1:500 |
| Antibody |
Secondary goat anti-rabbit |
Abcam AlexaFluor 488 |
#ab150077 |
1:500 |
| Recombinant DNA reagent |
Cas9-mSA plasmid |
PMID:29889212
|
|
|
| Recombinant DNA reagent |
Cas9 no-mSA plasmid |
PMID:29889212
|
|
|
| Recombinant DNA reagent |
Cas9 plasmid |
PMID:24873830
|
|
|
| Sequence-based reagent |
gRNA actb
|
Sigma |
|
Sequence (spacer)GGAUGAUGACAUUGCCGCAC |
| Sequence-based reagent |
gRNA cbx1b
|
Sigma |
|
Sequence (spacer)GGAAGAUGUGGCAGAAGAAG |
| Sequence-based reagent |
gRNA cdh2
|
Sigma |
|
Sequence (spacer)GGGAGCGAUGACUAAGACAA |
| Sequence-based reagent |
gRNA g3bp1
|
Sigma |
|
Sequence (spacer)CCCCAGCGAGAGCCGCUUCU |
| Sequence-based reagent |
gRNA mapre1b
|
Sigma |
|
Sequence (spacer)UCCAGAUGCUGAGGAACAGG |
| Sequence-based reagent |
gRNA myosinhc
|
Sigma |
|
Sequence (spacer)CAUCUCUGCGUCAGUGCUCA |
| Sequence-based reagent |
gRNA pcna
|
Sigma |
|
Sequence (spacer)GACCAGGCGAGCCUCAAACA |
| Sequence-based reagent |
gRNA rab11a
|
Sigma |
|
Sequence (spacer)UCGGAUUAACGCGAGGACGA |
| Commercial assay or kit |
RNAeasyMiniKit |
Qiagen |
#74104 |
|
| Commercial assay or kit |
QIAquickGel Extraction Kit |
Qiagen |
#28115 |
|
| Commercial assay or kit |
mMachineSP6 Transcription Kit |
Invitrogen |
#AM1340 |
|
| Software, algorithm |
CC-Top |
PMID:25909470
|
|
|
| Software, algorithm |
Ensembl |
Public |
|
|
| Chemical compound, drug |
Hoechst 33342 |
Thermo Fischer |
#H3570 |
1:500 dilution of the 10 mg/ml stock solution |
| Chemical compound, drug |
Tricane |
Sigma-Aldrich |
#A5040-25G |
|
| Chemical compound, drug |
LMP Agarose |
Biozyme |
Plaque Agarose #840,101 |
0.6% in 1× H2O |
