Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Danio rerio) | Tg(lratd2a:QF2)c601 | This paper | Transgenic,Halpern lab | |
| Genetic reagent (Danio rerio) | Tg(slc5a7a:Cre)c662 | This paper | Transgenic,Halpern lab | |
| Genetic reagent (Danio rerio) |
Tg(Xla.Tubb2:QF2; he1.1:mCherry)c663 |
This paper | Transgenic,Halpern lab | |
| Genetic reagent (Danio rerio) | Tg(QUAS:GCaMP6f)c587 | This paper | Transgenic,Halpern lab | |
| Genetic reagent (Danio rerio) | Tg(QUAS:GFP)c403 | Subedi et al., 2014 | Transgenic,Halpern lab | |
| Genetic reagent (Danio rerio) | Tg(QUAS:mApple-CAAX;he1.1:mCherry)c636 | This paper | Transgenic,Halpern lab | |
| Genetic reagent (Danio rerio) | Tg(QUAS:loxP-mCherry-loxP-GFP-CAAX)c679 | This paper | Transgenic,Halpern lab | |
| Genetic reagent (Danio rerio) | Tg(QUAS:loxP-mCherry-loxP-BoTxBLC-GFP)c674 | This paper | Transgenic,Halpern lab | |
| Genetic reagent (Danio rerio) | Tg(–10lhx2a:gap-EYFP)zf177 | Miyasaka et al., 2009 | RRID:ZFIN_ZDB-GENO-100504-13 | Transgenic |
| Genetic reagent (Danio rerio) | Tg(lhx2a:syp-GFP)zf186 | Miyasaka et al., 2009 | RRID:ZFIN_ZDB-GENO-100504-13 | Transgenic |
| Genetic reagent (Danio rerio) | tcf7l2zf55 | Muncan et al., 2007 | RRID:ZFIN_ZDB-GENO-071217-3 | Mutant |
| Genetic reagent (Danio rerio) | bsxm1376 | Schredelseker and Driever, 2018 | RRID:ZFIN_ZDB-GENO-180802-1 | Mutant |
| Chemical compound, drug | alpha-Bungarotoxin | Invitrogen | Cat# B-1601 | (1 mg/ml) |
| Chemical compound, drug | Cadaverine | Sigma-Aldrich | Cat# 33,211 | (100 μM) |
| Chemical compound, drug | Chondroitin sulfate sodium salt from shark cartilage | Sigma-Aldrich | Cat# C4384 | (100 μg/ml) |
| Chemical compound, drug | T7 Endonuclease I | NEB | Cat# M0302L | |
| Chemical compound, drug | MAXIscript T7 Transcription Kit | Invitrogen | Cat# AM1312 | |
| Chemical compound, drug | mMASSAGE mMACHINE T3 Transcription Kit | Invitrogen | Cat# AM1348 | |
| Chemical compound, drug | Gateway BP Clonase II Enzyme mix | Thermo Fisher Scientific | Cat# 11789020 | |
| Chemical compound, drug | Gateway LR Clonase II Enzyme mix | Thermo Fisher Scientific | Cat# 11791020 | |
| Chemical compound, drug | DIG RNA Labeling Mix | Roche | Cat# 11277073910 | |
| Chemical compound, drug | 5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt (BCIP) | Roche | Cat# 11383221001 | |
| Chemical compound, drug | 4-Nitro blue tetrazolium chloride, solution (NBT) | Roche | Cat# 11383213001 | |
| Chemical compound, drug | 2-(4-Iodophenyl)–3-(4-nitrophenyl)–5-phenyltetrazolium Chloride (INT) | FisherScientific | Cat# I00671G | |
| Antibody | Anti-Digoxigenin-AP, Fab fragments antibody(Sheep polyclonal) | Roche | Cat# 11093274910, | (1:5000) |
| Antibody | Anti- Fluorescein -AP, Fab fragments antibody(Sheep polyclonal) | Roche | Cat# 11426338910 | (1:5000) |
| Recombinant DNA reagent | Plasmid: Gbait-hs-Gal4 | Kimura et al., 2014 | N/A | |
| Recombinant DNA reagent | Plasmid: NBeta-pEGFP-1 | Gift fromPaul Krieg | N/A | |
| Recombinant DNA reagent | Plasmid: Gbait-hsp70-QF2-SV40pA | This paper | Addgene Plasmid#122,563 | Donor plasmid for integration,Halpern lab |
| Recombinant DNA reagent | Plasmid: Gbait-hsp70-Cre-SV40pA | This paper | Addgene Plasmid#122,562 | Donor plasmid for integration,Halpern lab |
| Recombinant DNA reagent | Plasmid: pDR274 | Hwang et al., 2013 | Addgene Plasmid#42,250 | |
| Recombinant DNA reagent | Plasmid: GFP sgRNA | Auer et al., 2014 | N/A | |
| Recombinant DNA reagent | Plasmid: pT3TS-nCas9n | Jao et al., 2013 | #46,757 | |
| Recombinant DNA reagent | pGEM-T Easy Vector | Promega | Catalog# A1360 | |
| Software, Algorithm | Fiji | Schindelin et al., 2012 | https://imagej.net/Fiji | |
| Software, Algorithm | MATLAB | The MathWorks | https://www.mathworks.com/ | |
| Software, Algorithm | Excel | Microsoft | http://products.office.com/en-us/excel | |
| Software, Algorithm | GraphPad Prism9 | GraphPad Software | https://www.graphpad.com/ | |
| Software, Algorithm | ZebraLab | Viewpoint Life Sciences | http://www.viewpoint.fr/en/home |