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. 2021 Dec 15;368(21-24):fnab156. doi: 10.1093/femsle/fnab156

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© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Membrane growth of S. moniliformis L-forms in a liquid medium. (A)Streptobacillus moniliformis cells in NB with serum were treated with 200 μg/ml ampicillin in the CellASIC ONIX microfluidic system for time-lapse microscopy. Individual frames are extracted from Video S4 (Supporting Information). Numbers in the top left corner of each frame represent time (min) elapsed in the video. Arrowheads represent spontaneous formation of L-form-like structures. Arrow represents OM expansion followed by growth and deformations of L-form cell. (B) Phase-contrast micrographs of S. moniliformis L-forms during time-lapse experiments. Individual frames are extracted from Video S5 (Supporting Information). Numbers in the top left corner of each frame represent time (min) elapsed in the video. Red arrows show expansion of OM. Blue arrows show growth and deformation of cytoplasmic compartment. Yellow arrowheads show scission events.